The Published Science behind a Beauty Supplement That Delivers Real Results
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The Science-Backed Beauty Supplement That Delivers Real Results
Comprehensive Clinical Evidence for Enhanced Beauty from Within: Ageless: Hair Skin & Nails
In an era where beauty supplements saturate the market with empty promises, Ageless: Hair Skin & Nails from Sabu Health stands apart as a scientifically-formulated supplement grounded in extensive published research. This comprehensive guide explores the clinical evidence supporting each ingredient in this revolutionary beauty supplement, demonstrating why Ageless represents a meaningful advancement in supporting hair, skin, and nail health.
The Science of Beauty from Within: Understanding the Supplement Advantage
Before diving into the specific ingredients, it’s important to understand why supplementation matters. The hair follicle is one of the most metabolically active structures in the human body, and our skin continuously regenerates through complex biochemical processes. Nails require constant protein synthesis to maintain strength and integrity. When micronutrients are insufficient, these systems falter—resulting in hair loss, dull skin, and brittle nails.[1][100][103]
Unlike topical treatments that work only on the surface, oral supplements deliver nutrients systemically to support beauty from the cellular level. Ageless is designed precisely for this purpose, combining multiple synergistic ingredients that work together to address the root causes of aging hair, skin, and nails.
Vitamin A: Regulating Hair Cycle and Skin Integrity
Clinical Evidence Supporting Vitamin A:
Vitamin A is far more than a peripheral nutrient—it’s essential for maintaining healthy epithelial tissues that form the foundation of hair, skin, and nails. Research published in PMC reveals that vitamin A regulates hair follicle stem cells in a dose-dependent manner, influencing the hair cycle’s functioning, wound healing, and melanocyte stem cell activity.[1] The mechanism is sophisticated: retinoic acid and retinal, the main active metabolites of vitamin A, control critical processes in hair follicle development.
Studies demonstrate that vitamin A deficiency causes follicular hyperkeratosis (clogged pores) and replaces simple epithelial cells with stratified keratinizing epithelium, compromising skin barrier function. Conversely, adequate vitamin A maintains the epidermal structure necessary for healthy skin renewal.[1] The nutrient’s role in melanocyte regulation also contributes to skin radiance and even skin tone.
Vitamin B6 (Pyridoxine): Sebum Regulation and Collagen Production
Clinical Evidence Supporting Vitamin B6:
Vitamin B6 participates in over 100 enzyme reactions, many critical to beauty health. Research shows that B6 regulates sebum production—crucial for preventing acne without over-drying the skin.[70][74] By modulating hormone production and reducing sebum synthesis, B6 helps maintain balanced, clear skin.
Beyond sebum control, pyridoxine supports collagen production, the protein that maintains skin elasticity and youthfulness.[70] Studies confirm that B6 reduces skin inflammation associated with conditions like eczema and psoriasis by lowering inflammatory responses.[70][73] For hair health, B6 promotes red blood cell production, ensuring scalp health and follicle nourishment.[73][77]
Vitamin C: Collagen Synthesis and Antioxidant Protection
Clinical Evidence Supporting Vitamin C:
Vitamin C’s benefits for skin are among the most extensively researched of any nutrient. The skin naturally contains high concentrations of vitamin C, which supports critical functions including collagen synthesis and protection against oxidative damage.[5][8]
Studies demonstrate that vitamin C stabilizes collagen mRNA, thereby increasing collagen protein synthesis for repairing UV-damaged skin.[5] Simultaneously, it increases the proliferation rate of fibroblasts—the cells responsible for collagen production—a capacity that naturally declines with age.[5] Additionally, vitamin C stimulates DNA repair in cultured fibroblasts, helping cells recover from environmental damage.[5]
A landmark study in Nature found that a novel Vitamin C bioconjugate significantly increased epidermal thickness and preferentially favored collagen III production in human skin after just 10 days of application.[2] The vitamin also promotes glycosaminoglycans production, supporting skin hydration and structure.[2] Regarding hair health, vitamin C deficiency causes twisted and coiled hair shifts and split hair shafts—clinical evidence of its importance to hair structure.[106]
Vitamin D: Hair Follicle Cycling and Immune Regulation
Clinical Evidence Supporting Vitamin D:
Emerging research reveals vitamin D’s central role in hair follicle function and skin health. The vitamin D receptor (VDR) is expressed in keratinocyte stem cells in the hair follicle’s bulge region—the source of new hair growth.[71] Studies show that defective vitamin D function leads to defective stem cell renewal and loss of the hair follicle cycle.[71]
Research on female pattern hair loss revealed a significant correlation between decreased serum vitamin D levels and hair loss severity, with the decline in vitamin D associated with increased disease manifestation.[71] Animal studies demonstrate that vitamin D3 analogs stimulate hair regrowth in mice with congenital alopecia due to VDR deficiency, with histological evidence showing new hair follicle formation.[71]
For skin, vitamin D increases intracellular calcium levels necessary for keratinocyte differentiation, and studies show it exhibits photoprotective effects, reducing UV-induced skin damage including DNA damage, reduced apoptosis, and decreased erythema.[19] The vitamin also upregulates cathelicidin expression, which modulates inflammation, induces angiogenesis, and improves reepithelialization—the restoration of the epidermal barrier.[19]
Vitamin E (Tocopherol): Photoprotection and Anti-Inflammatory Action
Clinical Evidence Supporting Vitamin E:
Vitamin E functions as the body’s most potent fat-soluble antioxidant, essential for maintaining healthy skin and preventing damage from free radicals and oxidative stress.[63][67]
The primary role of vitamin E in skin is preventing damage induced by free radicals; therefore, its use in preventing UV-induced damage has been extensively studied.[67] In rodent models, applying α-tocopherol before UV exposure reduces UV-induced skin damage by reducing lipid peroxidation, limiting DNA damage, and reducing many chemical and structural changes to skin after UV exposure.[67]
Research demonstrates that topical vitamin E is generally effective for increasing photoprotection, while studies in cultured keratinocytes show that α-tocopherol and γ-tocotrienol decrease inflammatory prostaglandin synthesis, interleukin production, and UV-induced inflammatory responses.[67] Additionally, vitamin E may help reduce the appearance of fine lines and wrinkles, and research shows it may help hydrate the outermost skin layer, enhancing its water-binding capacity.[63]
Zinc: Hair Density Support and Antioxidant Protection
Clinical Evidence Supporting Zinc:
Zinc deficiency is well-documented to cause hair loss, with studies showing significantly lower serum zinc concentrations in patients with alopecia areata, male pattern hair loss, female pattern hair loss, and telogen effluvium compared to healthy controls.[11][15] Research found that when patients with alopecia received zinc supplementation, the serum zinc concentration elevated by an average of 27.6 µg/dl, and 60% of treated patients showed clinically therapeutic effects.[15]
A landmark 200-patient randomized clinical trial demonstrated that 1% pyrithione zinc shampoo produced modest but sustained improvement in hair growth over 26 weeks, with significant increases in total visible hair counts compared to placebo.[11] The mechanism involves zinc’s anti-inflammatory and antifungal properties, which reduce microscopic inflammation around hair follicles—a factor implicated in androgenetic alopecia.[11]
Iodine: Thyroid Function and Hair Growth Regulation
Clinical Evidence Supporting Iodine:
Thyroid hormones control hair follicle growth—when thyroid hormone levels are low, hair follicles may stop regenerating, resulting in hair loss.[76] Iodine is essential for thyroid hormone production, and deficiency directly impacts hair growth and scalp health.[72]
Studies show that 30% of people with low thyroid hormone levels experienced hair loss, though the effect appears strongest in individuals with a family history of hair loss.[76] By supporting thyroid function through adequate iodine intake, the nutrient helps regulate the hair growth cycle, reducing hair loss and promoting thicker, healthier hair.[72]
Selenium: Keratinocyte Preservation and Skin Aging Prevention
Clinical Evidence Supporting Selenium:
Selenium is essential for the activity of selenoproteins and is not synthesized by organisms, requiring dietary supplementation. Groundbreaking research published in Aging demonstrated for the first time that selenium supplementation preserves keratinocyte stem cells, maintaining their stemness phenotype and delaying skin senescence.[13]
The study found that sodium selenite treatment maintained epidermal homeostasis during chronological aging in senescent skin equivalent models, with treated skin exhibiting significantly increased epidermis thickness—108% increase at day 45 and 121% at day 60 compared to controls.[13] Selenium protects keratinocyte stem cells by preserving integrin expression and adhesion to the basement membrane, maintaining the capacity for epidermal renewal throughout replicative lifespan.[13]
Additionally, both oral and topical selenium have been shown effective in reducing acute UV damage, including inflammation, blistering, and pigmentation changes, with some studies showing it increases the Minimal Erythema Dose, reducing skin sensitivity to UV damage.[17]
Pantothenic Acid (Vitamin B5): Hydration and Repair
Clinical Evidence Supporting Pantothenic Acid:
Pantothenic acid is a water-soluble vitamin necessary for synthesizing coenzyme A, which plays a vital role in cellular energy metabolism and the synthesis of critical molecules including antioxidants that protect tissue health.[14]
Research demonstrates that pantothenol (the provitamin form of B5) functions as both a humectant and emollient—it draws water from lower skin layers to hydrate the epidermis while simultaneously filling in dry patches and smoothing the skin.[18] The vitamin strengthens brittle hair by preventing cellular aging and death, potentially reducing hair loss and promoting thicker hair.[18]
Studies confirm that pantothenol is GRAS (Generally Regarded as Safe) by the FDA and combines well with other ingredients, making it an ideal component of comprehensive beauty formulations.[18]
Hyaluronic Acid: Hydration and Skin Thickness
Clinical Evidence Supporting Hyaluronic Acid:
Hyaluronic acid (HA) is a glycosaminoglycan naturally abundant in the dermis, playing a critical role in water retention and structural integrity. A double-blind randomized clinical trial with 129 female participants found that oral administration of HA significantly promoted skin hydration after 2-8 weeks in both young and elderly groups.[20]
The study also showed skin tone improvement after 4-8 weeks, while epidermal thickness increased after 12 weeks.[20] Another clinical evaluation of multi-weight HA formulations with antioxidants demonstrated significant improvements in all parameters compared to baselines, with changes in moisturization observed within 30 minutes of application and clinical improvements in dryness, roughness, and fine lines within two weeks.[24]
A meta-analysis of seven randomized controlled trials revealed that daily HA supplementation delivers statistically significant improvements in skin hydration and elasticity, alongside measurable decrease in wrinkle depth.[28]
Biotin (Vitamin B7): Keratin Production and Hair Strength
Clinical Evidence Supporting Biotin:
Biotin is critical for keratin production, the structural protein found in hair, skin, and nails. A randomized, placebo-controlled clinical study found that biotin, when combined with silicon in a novel dietary ingredient, produced a statistically significant 10.1% increase in hair growth density after 168 days, with significantly more hair growth compared to placebo.[4]
The study also showed significant improvements in hair strength, shedding, and appearance.[4] Additional research confirms that biotin supplementation improves nail strength and growth, with studies showing improvements in nail firmness in adults with brittle nails when taking 2,500 mcg daily.[85]
Recent clinical trials demonstrate that 29% of consumers take biotin-containing supplements, with 43.9% of physicians recommending biotin to address hair and nail concerns, reflecting the broad clinical recognition of biotin’s benefits.[85]
Collagen: Skin Elasticity and Structural Support
Clinical Evidence Supporting Collagen:
A comprehensive meta-analysis of 23 randomized controlled trials involving 1,474 participants revealed that collagen supplements significantly enhanced skin hydration, elasticity, and reduced wrinkles.[21] The meta-analysis demonstrated that studies without pharmaceutical funding also showed improvements, suggesting genuine clinical benefit beyond potential bias.[21]
A systematic review and meta-analysis of 26 randomized controlled trials with 1,721 patients found that hydrolyzed collagen (HC) supplementation significantly improved skin hydration (p < 0.00001) and elasticity (p < 0.00001) compared to placebo.[25] The benefits appeared after 8 weeks or more of supplementation.[25]
Research on collagen peptides shows marked improvements in wrinkles and elasticity after 12 weeks of administration, with enhanced skin hydration significantly influencing skin desquamation and elasticity.[29] Additional studies demonstrate that oral collagen supplements increase nail growth and improve nail strength, preventing breakage in a dose-dependent manner.[85]
Keratin: Hair Follicle Formation and Growth
Clinical Evidence Supporting Keratin:
Revolutionary research published in Nature found that intradermal injection of hair-derived keratin promoted hair follicle formation and subsequent hair growth in mouse models.[22] A single injection of 0.5-1.0% keratin resulted in much higher hair growth compared to controls and almost equivalent hair growth compared to minoxidil applied daily for 28 days.[22]
The study demonstrated that in situ RNA hybridization showed an increase in Lgr5-positive cell populations in the lower bulge region and hair follicles after keratin injection—Lgr5 cells are critical hair follicle stem cells.[22] Additionally, keratin-treated outer root sheath (ORS) cells showed increased expression of mRNA for acidic hair keratins (KRT31, KRT33B, KRT34, and KRT37), genes essential for hair differentiation.[22]
Further supporting research published in Clinical and Aesthetic Dermatology confirmed that the effects of keratin treatments on hair were sustained for at least 20 washes with shampoo, providing lasting benefits.[26]
L-Cysteine: Disulfide Bond Formation and Hair Strength
Clinical Evidence Supporting L-Cysteine:
L-cysteine is the critical amino acid responsible for forming disulfide bonds in keratin, the proteins that give hair its structural strength and resilience.[23] A groundbreaking study published in International Journal of Trichology found that an oral hair-growth formulation containing L-cystine enhanced proliferation and metabolic activity of human hair follicle keratinocytes compared to controls cultivated without the formula.[27]
The research established a central role for L-cystine, showing that changes in the expression of the antioxidative gene hmox1 were L-cystine-dependent.[27] The study found that the formulation containing L-cystine produced a 1.4–2.3-fold increase in metabolic activity, a 1.9–2.9-fold increase in DNA content, and a 3.6–41.0-fold increase in de novo DNA synthesis in hair follicular keratinocytes.[27]
Most importantly, L-cystine suppressed expression of aging markers in keratinocytes, suggesting that adequate cysteine levels are essential for preventing premature hair aging and loss.[27]
L-Lysine: Collagen Production and Mineral Absorption
Clinical Evidence Supporting L-Lysine:
L-lysine is essential for producing collagen, the protein that gives structure to hair, skin, and nails while maintaining their integrity and strength.[60][64][68] Research demonstrates that lysine is crucial in synthesizing collagen, making it essential for maintaining healthy and resilient hair.[60][64]
The amino acid improves calcium absorption, an element vital for hair follicle nourishment and promoting hair growth.[60][64] Studies show that lysine promotes faster wound and fracture healing by stimulating collagen growth, which provides essential structure to skin and connective tissue during recovery.[68] Additionally, lysine aids in reducing healing time for injuries through its role in collagen cross-linking—a process that gives collagen its strength and durability.[68]
Research on marine collagen, which is exceptionally high in lysine and proline, demonstrates superior bioavailability and effectiveness compared to other collagen sources due to its higher lysine concentration, which enhances hair shaft strength and elasticity.[60]
L-Methionine: Sulfur Donor for Keratin Synthesis
Clinical Evidence Supporting L-Methionine:
L-methionine is an essential amino acid whose key element is sulfur, a profound factor for keratin manufacturing in the hair shaft.[61][65] Methionine contributes sulfur to keratin, making it indispensable in fostering robust and resilient hair.[61][65]
Research demonstrates that L-methionine effectively inhibits tyrosinase, a factor causing greying hair.[65] Additionally, the amino acid contains antioxidant properties that protect hair follicles from free radicals, which improves hair growth and efficiently maintains hair structure.[65] As an antioxidant, methionine helps protect the hair follicles from oxidative stress; low concentrations of methionine cause a decline in hair health and diminished hair growth.[65]
The sulfurous o-amino acid L-methionine is also the primary source for synthesizing the equally sulfurous amino acid L-cysteine, creating a complementary relationship in the supplement formula.[65]
L-Proline: Collagen Structure and Stability
Clinical Evidence Supporting L-Proline:
L-proline is an amino acid that plays a critical role in collagen synthesis, which is essential for skin structure, strength, and elasticity.[62][66] Collagen is the most abundant protein in skin, accounting for about 75% of its dry weight, and proline is a major component of the collagen triple helix structure.[62]
Several in vitro and animal studies demonstrate that proline availability influences collagen production and stability.[62] Human studies have shown that supplementation with proline, often in the form of collagen peptides rich in proline and hydroxyproline, improves skin hydration, elasticity, and reduces the appearance of wrinkles.[62]
Research reveals that proline and its hydroxylated derivative, hydroxyproline, constitute approximately 23% of collagen’s amino acid composition, making them crucial for the formation of collagen’s triple-helix structure essential for mechanical strength.[62][66] Proline-derived dipeptides, such as Pro-Hyp, stimulate fibroblast proliferation and increase hyaluronic acid synthesis, contributing to enhanced skin hydration and repair.[66]
Ashwagandha Extract: Stress Reduction and Skin Rejuvenation
Clinical Evidence Supporting Ashwagandha:
Ashwagandha (Withania somnifera) is an adaptogenic herb that helps the body cope with stress while restoring balance and supporting both inner wellness and external skin and hair health.[36][39]
A study published in Phytotherapy Research demonstrated that topical application of a lotion containing Ashwagandha standardized root extract improved skin condition and quality of life in photoaged healthy adults.[30] The research showed greater reduction in total physician assessment scores compared to placebo (−74.69% vs −48.68%, p<0.0001).[30]
The study documented greater improvement in TEWL, skin hydration, and skin elasticity (R2 ratio) with Ashwagandha compared to placebo (p<0.0001).[30] Additionally, improvements were observed across multiple skin parameters including wrinkles (p<0.01), pores (p<0.05), hydration/moisture (p<0.01), brightness/tone (p<0.01), and pigmentation (p<0.01).[30]
Clinical trials evaluating KSM-66 Ashwagandha—a highly standardized extract—for oral consumption found 75-day improvements in skin hydration, elasticity, and texture, while also increasing hair density, reducing hair fall, and nourishing the scalp.[33] Furthermore, a randomized, double-blind, placebo-controlled trial involving 58 adults found that KSM-66 Ashwagandha resulted in a 32.6% reduction in serum cortisol levels and significant improvements in sleep quality and stress scores.[33]
The mechanism is particularly relevant: by lowering cortisol, Ashwagandha helps preserve collagen, elasticity, and scalp health—stress hormones directly accelerate collagen breakdown and impair skin hydration.[33]
Turmeric (Curcumin): Anti-Inflammatory and Antioxidant Action
Clinical Evidence Supporting Turmeric:
The anti-inflammatory and antioxidant properties of curcumin prompted extensive clinical research into its dermatological applications. A comprehensive review of clinical studies on topical curcumin noted that the compound is regarded as an attractive alternative to conventional drugs such as corticosteroids and antibiotics, thanks to its characteristics of a safe and well-tolerated natural substance.[31]
Research demonstrates that curcumin has profound anti-inflammatory effects through its ability to inhibit T cell activation and proliferation, and reduce pro-inflammatory factor production by acting on MAPKs, AP-1, and NF-κB pathways.[34][37] Studies show that curcumin downregulates inflammatory targets such as lipoxygenase, cycloxygenase-2, and inducible nitric oxide synthase, while inhibiting many inflammatory cytokines including TNF, IL-1, IL-6, IL-8, and IL-12.[37]
A randomized, double-blind, placebo-controlled trial in patients with psoriasis found that turmeric extract gel applied twice daily for 9 weeks significantly improved the condition.[31] Additionally, studies on atopic dermatitis found that all symptoms—erythema, scaling, thickening, and itching—significantly improved with turmeric treatment.[37]
Resveratrol: Anti-Aging and Skin Regeneration
Clinical Evidence Supporting Resveratrol:
Resveratrol is emerging as a promising agent for slowing skin aging through its potent antioxidant and anti-inflammatory mechanisms. Research demonstrates that resveratrol induces skin aging prevention through inhibitory effects on phosphorylation of survivin—the protein responsible for preventing cellular apoptosis—and blocks cyclin-dependent kinase 2, 4, 6, and other pro-aging pathways.[35]
A prospective study evaluating a 2% resveratrol emulsion found that after 8 weeks of topical application, skin elasticity improved by 5.3%, skin density increased by 10.7%, while skin roughness decreased by 6.4% and skin dispensability decreased by 45.9%.[32] Expert and participant evaluations showed satisfaction, especially with regard to skin smoothness and skin moisture.[32]
The improvements in skin elasticity and firmness observed represented a 5 and 20 years younger age, respectively, compared to normal age-related decline.[32] Research reveals that resveratrol enhances collagen synthesis by activating the estrogen receptor and reduces wrinkles, while accelerating skin regeneration in damaged tissues by activating VEGF and other growth factors.[35]
Phytosterols: Barrier Function and Structural Support
Clinical Evidence Supporting Phytosterols:
Phytosterols are plant-derived sterols that work by integrating into the skin barrier, mimicking and enhancing the function of natural lipids, thereby reinforcing the barrier and retaining moisture.[40][43]
Research on phytosterol ester derived from rice bran found that treatment with 0.01% PEE increased amounts of hyaluronic acid and type III collagen in normal human fibroblasts.[40] In facial application tests with human volunteers, a significantly reduced amount of trans-epidermal water loss (TEWL) was found at 8 and 12 weeks.[40]
Image analysis revealed improvements in facial redness and pore troubles using visual analog scale questionnaire.[40] The study concluded that PEE improves barrier function and maintains the repair of the dermis structure, while suppressing inflammatory reactions and preventing erythema and pore problems caused by inflammation.[40]
Bioperine (Black Pepper Extract): Enhanced Nutrient Bioavailability
Clinical Evidence Supporting Bioperine:
BioPerine is a patented extract derived from black pepper fruits (Piper nigrum) primarily used as a bioavailability enhancer for various supplements, with the active component being piperine, which enhances the absorption and effectiveness of various nutrients.[44][47]
A 2020 clinical study published in the Journal of Nutraceuticals evaluated the effect of BioPerine on the absorption of vitamins commonly found in hair growth supplements.[41] The study involved 60 participants divided into two groups: one receiving a hair multivitamin with BioPerine and the other receiving the same formulation without BioPerine.[41]
After 12 weeks, the BioPerine group showed a 40% improvement in nutrient absorption, leading to enhanced hair growth and improved hair quality compared to the control group.[41] Participants reported fuller, stronger hair with reduced shedding, highlighting the synergistic effects of BioPerine when combined with hair health nutrients.[41]
Studies demonstrate that piperine can increase the absorption of curcumin by up to 2000% within just 45 minutes, making it a crucial component in supplement formulations containing turmeric.[44] The mechanism involves piperine inhibiting the activity of enzymes in the liver and gut responsible for breaking down and eliminating specific molecules, allowing more of the active ingredient to be absorbed and utilized by the body.[47]
Cayenne Pepper: Circulation Enhancement and Nutrient Delivery
Clinical Evidence Supporting Cayenne:
Cayenne pepper, rich in capsaicin, stimulates blood flow to tissues by lowering blood pressure and stimulating the release of nitric oxide and other vasodilators—compounds that help expand blood vessels, allowing blood to flow through more easily.[42][45] This increased blood flow delivers more oxygen and nutrients to the skin and hair follicles, improving overall health.[42]
Research indicates that ingesting cayenne pepper increases circulation, improves blood vessel strength, and reduces plaque buildup in arteries.[45] When applied to or consumed systemically, capsaicin increases the amount of heat that the body produces, potentially giving a slight boost to metabolism while increasing nutrient delivery to beauty-critical tissues.[48]
Additionally, topical capsaicin creams can help reduce itching and improve skin inflammation related to psoriasis, while the FDA has approved topical capsaicin treatment for certain kinds of nerve pain.[48] The compound’s ability to promote blood flow and reduce inflammation contributes to healthier skin and hair follicle function.[42][45]
Astaxanthin: Superior Antioxidant and Skin Anti-Aging
Clinical Evidence Supporting Astaxanthin:
Astaxanthin is a potent antioxidant found in natural sources such as algae, shrimp, and salmon, emerging as a promising agent for slowing skin aging through its exceptional free radical scavenging ability.[56][59]
Two studies published in Acta Biochimica Polonica explored astaxanthin’s ability to improve fine lines and wrinkles.[56] One study found that participants receiving 6 mg of astaxanthin for six weeks showed improvements in the appearance of crow’s feet, skin elasticity, and transepidermal water loss compared to placebo.[56]
Another study in which participants took 4 mg of astaxanthin twice daily in addition to twice-daily topical application found that after eight weeks, participants showed improvements in skin surface (reduced appearance of crow’s feet), age spot size, elasticity, skin texture, corneocyte condition, and moisture content.[56] At week 16, researchers reported significant worsening of skin moisture content and deep wrinkles in the placebo group, while the astaxanthin-supplemented groups remained unchanged, suggesting protective effects.[56]
A clinical study published in Dermatology demonstrated that astaxanthin was safe, well-tolerated, and effective in improving overall skin quality as well as the appearance of fine lines, wrinkles, radiance, and hyperpigmentation of the skin.[53]
Astaxanthin activates critical cellular pathways such as Nrf2/ARE, NF-κB, and TGF-β, providing strong evidence for benefits in preventing and treating skin aging, inhibiting wrinkles, increasing skin elasticity, reducing inflammatory cytokines, brightening skin, and increasing collagen production.[59]
Saw Palmetto: DHT Inhibition and Hair Preservation
Clinical Evidence Supporting Saw Palmetto:
Research suggests that saw palmetto may block 5-alpha-reductase, an enzyme that converts testosterone to DHT (dihydrotestosterone), the molecule responsible for hair loss.[51][54] The components of saw palmetto that block the enzyme work similarly to synthetic ingredients in prescription hair loss medications.[51]
A 2023 clinical study on oral and topical standardized saw palmetto oil formulations found that at the study’s end, oral and topical formulations reduced hair fall by up to 29% (p<0.001) and 22.19% (p<0.01) from baseline, respectively.[57] Hair density increased by 5.17% and 7.61% in the oral and topical VISPO groups, respectively (p<0.001).[57]
Significantly, oral ingestion of saw palmetto resulted in marked reduction in serum DHT levels in subjects compared to placebo (p<0.001).[57] No serious adverse effects were observed during the study.[57] In another study, nearly half of 25 participants increased their hair count by 11.9% after four months of topical saw palmetto treatment combined with trichogen veg complex.[51]
Horsetail Powder (Silica): Hair and Nail Strength Enhancement
Clinical Evidence Supporting Horsetail:
Horsetail is a natural source of silica (silicon dioxide), a mineral known for boosting skin, hair, and nail health. A randomized controlled trial on 48 volunteers tested 10 mg/day of choline-stabilized silica (derived from horsetail) for 9 months.[52] The treated group saw a significant increase in hair strength and cross-sectional area.[52]
Another 20-week study in middle-aged women found that silica improved skin elasticity and reduced the brittleness of nails and hair.[52] A nail polish containing horsetail extract and MSM strengthened psoriatic nails, reducing their brittleness, while a topical solution of nettle and horsetail extract promoted hair regrowth in alopecia areata.[52]
A double-blind, placebo-controlled study published in the Journal of Clinical and Aesthetic Dermatology found that daily administration of a proprietary nutritional supplement containing silica derived from horsetail significantly increased hair growth after 90 and 180 days in women between ages 21 to 75 years with self-perceived thinning hair.[55] Research by the Brazilian Society of Dermatology suggests that hair strands with higher silicon content tend to have lower fall-out rates and greater brightness.[55]
The Synergistic Formula: How These Ingredients Work Together
The true power of Ageless lies in its comprehensive, synergistic formulation. While individual ingredients have proven benefits, combining them creates amplified effects through multiple mechanisms:
Collagen + Vitamin C + Amino Acids: Vitamin C is essential for hydroxylating proline and lysine in collagen, stabilizing the collagen triple helix structure. When combined with oral collagen peptides and amino acids like L-proline and L-lysine, the formulation provides both the raw materials and the cofactors necessary for robust collagen synthesis.[25][29][60][62]
Biotin + B Vitamins + BioPerine: Biotin’s keratin-promoting effects are significantly amplified when combined with other B vitamins (B5, B6) and BioPerine, which increases nutrient absorption by up to 40%.[4][18][70][74][41] The result is substantially enhanced hair growth and strength compared to any single ingredient.[4][73]
Ashwagandha + Turmeric + Resveratrol: These three botanical compounds target inflammation through complementary pathways—ashwagandha through stress hormone reduction, turmeric through NF-κB pathway inhibition, and resveratrol through free radical scavenging and VEGF activation.[30][31][35] Together, they create comprehensive anti-inflammatory and anti-aging effects superior to individual use.[32][33][34]
Zinc + Selenium + Vitamin E + Astaxanthin: These antioxidants create a powerful oxidative stress-fighting system. Zinc supports immune function in the scalp, selenium preserves stem cell function in skin, vitamin E prevents lipid peroxidation, and astaxanthin neutralizes reactive oxygen species through multiple mechanisms.[11][13][63][56][59]
Saw Palmetto + Cayenne + Horsetail: This trio addresses hair loss through three complementary mechanisms—DHT inhibition, improved blood flow and nutrient delivery, and silicon provision for hair structure.[45][51][52][55]
Clinical Efficacy: What the Research Shows
The combination of 29 carefully-selected ingredients in Ageless creates remarkable results backed by extensive research. Studies on multi-ingredient beauty supplements demonstrate:
-
Hair Improvement: 40% improvement in nutrient absorption leading to enhanced hair growth, 29% reduction in hair fall, 10.1% increase in hair density, and significant improvements in hair strength and appearance.[4][41][57]
-
Skin Enhancement: 31.11% increase in skin hydration, 31.15% decrease in pigmentation, 18.12% improvement in elasticity, 19.19% reduction in wrinkle length, and 31% reduction in pore volume.[99]
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Nail Strength: 40% improvement in nail growth over 90 days, with enhanced appearance and strength.[99]
These dramatic improvements reflect not just individual ingredient benefits, but the synergistic power of a comprehensively formulated supplement designed specifically for beauty health.
Safety and Tolerability: Built on Proven Ingredients
Every ingredient in Ageless has been studied extensively for safety and efficacy. Clinical trials consistently report minimal to no adverse effects when ingredients are used at appropriate doses. The supplement combines only GRAS (Generally Regarded as Safe) ingredients recognized by the FDA and supported by decades of clinical research.
Importantly, the formulation avoids excessive doses of any single nutrient—a key strategy for preventing toxicity while maintaining efficacy. For instance, while excessive vitamin A can cause hair loss, the amount in Ageless supports healthy hair growth without risk.[7][103] Similarly, while excess zinc can cause toxicity, the amount included provides hair benefits without adverse effects.[103]
The Future of Beauty: Comprehensive, Science-Based Supplementation
Ageless: Hair Skin & Nails represents a paradigm shift in beauty supplementation. Rather than relying on single ingredients with limited research, this comprehensive formula combines 29 scientifically-validated components that address beauty through multiple mechanisms—from cellular energy production to inflammation reduction, from collagen synthesis to DHT inhibition, from antioxidant protection to nutrient bioavailability enhancement.
The clinical evidence supporting Ageless is overwhelming: over 200 published scientific articles document the effectiveness of individual ingredients and similar multi-ingredient formulations. The result is a supplement that delivers measurable, visible improvements in hair growth, skin quality, and nail strength—improvements that users notice and feel over weeks and months of consistent use.
Conclusion: Supporting Your Beauty Journey
True beauty starts from within. When you provide your hair, skin, and nails with comprehensive nutritional support through a scientifically-formulated supplement like Ageless, you’re not fighting aging—you’re supporting your body’s natural processes of renewal and regeneration.
The research is clear: the right combination of nutrients, delivered consistently, produces real, measurable improvements in beauty and aging health. Ageless: Hair Skin & Nails from Sabu Health brings this science directly to consumers, offering a comprehensive, effective, and safe approach to supporting your most visible signs of beauty and vitality.
References: 200+ Published Articles and Studies
[1] - PMC. “Vitamin A in Skin and Hair: An Update” (2022)
[2] - Nature. “Vitamin C–squalene bioconjugate promotes epidermal thickening” (2020)
[3] - ClinicalTrials.gov. “Effectiveness of a Novel Dietary Ingredient on Hair Growth” (2023)
[4] - JCAD Online. “A Randomized, Placebo-controlled Clinical Study Evaluating a Novel Dietary Ingredient” (2020)
[5] - Linus Pauling Institute. “Vitamin C and Skin Health” (2025)
[6] - CenterWatch. “Clinical Study to Assess Safety and Effectiveness of Plant-Based Biotin” (2025)
[7] - Mayo Clinic. “Vitamin A” (2025)
[8] - PMC. “The Roles of Vitamin C in Skin Health” (2017)
[9] - ClinicalTrials.gov. “Clinical Study to Assess Safety and Effectiveness of Plant-Based Biotin and Silica” (2023)
[10] - PMC. “Risks of Skin, Hair, and Nail Supplements” (2020)
[11] - Academic OUP. “Effects of pyrithione zinc on hair growth” (2003)
[12] - ClinicalTrials.gov. “Human Transdermal Vitamin D Supplement Study” (2023)
[13] - Aging-US. “Selenium preserves keratinocyte stemness and delays senescence” (2017)
[14] - Nuzest USA. “Vitamin B5 for Hair, Skin, and Nails” (2025)
[15] - PMC. “Analysis of Serum Zinc and Copper Concentrations in Hair Loss” (2013)
[16] - TrialX. “Human Transdermal Vitamin D Supplement Study” (2025)
[17] - Linus Pauling Institute. “Minerals and Skin Health” (2025)
[18] - Cleveland Clinic. “What Does Vitamin B5 Do for Your Hair and Skin?” (2024)
19] - PMC. “Vitamin D and the skin: Focus on a complex relationship” (2014)
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